TABLE 2.
BFA treatment reduces HLA-DR surface expression of stimulated APCsa
| MFI of HLA-DR-positive APCs treated with:
| ||||||
|---|---|---|---|---|---|---|
| Medium | Sp1 | Sp1+BFA | TT | TT+BFA | SEA | SEA+BFA |
| 236 | 924 | 237 | 970 | 355 | 570 | 200 |
a
γ-Irradiated mononuclear cells were treated with Sp1 (50 μg/ml), TT (2Lf/ml), and SEA (10 ng/ml) for 8 h at 37°C in the absence or presence of 100 nM BFA. Surface expression of HLA-DR was then assessed by flow cytometry as described in Materials and Methods. This result is representative of three independent experiments.