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Conversion of SL3261 to SLDAPD and plasmid selection by ORT. (A) Wild-type dapD locus in SL3261. (B) Replacement of dapD with the lacO/P-dapD-lacIq cassette to create SLDAPD. Induction with IPTG is required for dapD expression in the absence of a plasmid. (C) When the organism is transformed with a plasmid containing a lacO sequence, the repressor is titrated and dapD is expressed.
