FIG. 2.
Colonization of spleens and Peyer's patches following oral inoculation of mice (n = 10) with SLDAPD or SL3261 carrying plasmid pUC18I. Mice were inoculated with 5 × 109 CFU of SLDAPD/pUC18I (circles), SL3261/pUC18I (triangles), or control strain SL3261 (squares) by using a gavage needle. Spleens (A) and Peyer's patches (B) were removed 8, 12, or 16 days after inoculation and homogenized in PBS before preparations were plated onto LB agar (solid symbols) or LB agar containing ampicillin (open symbols) for enumeration of bacteria.