Table 2.
Results of NormFinder for the selection of reference gene/s for normalisation of RT-qPCR results
| Gene name | Stability value | ||
|---|---|---|---|
| miR-16-5p | 0.023 | ||
| miR-191-5p | 0.012 | ||
| miR-484 | 0.013 | ||
| SNORD96A | 0.016 | ||
| U6 snRNA | 0.024 | ||
| Average (miR-16-5p, miR-191-5p, miR-484) | 0.007 | ||
| Average (miR-16-5p, miR-191-5p, SNORD96A) | 0.014 | ||
| Average (miR-16-5p, miR-191-5p, U6 snRNA) | 0.004 | ||
| Average (SNORD96A, miR-191-5p, U6 snRNA) | 0.006 | ||
| Average (miR-191-5p,miR-484, SNORD96A) | 0.004 | ||
| Average (miR-191-5p,miR-484, U6 snRNA) | 0.011 | ||
| Average (miR-484, SNORD96A,U6 snRNA) | 0.011 | ||
| Intragroup variation | |||
| Group identifier | OPMD | Controls | Oral cancer |
| miR-16-5p | 0.004 | 0.003 | 0.002 |
| miR-191-5p | 0.001 | 0.001 | 0.001 |
| miR-484 | 0.001 | 0.001 | 0.001 |
| SNORD96A | 0.003 | 0.002 | 0.003 |
| U6 snRNA | 0.004 | 0.004 | 0.005 |
| Average (miR-16-5p, miR-191-5p, miR-484) | 0.001 | 0.000 | 0.000 |
| Average (miR-16-5p, miR-191-5p, SNORD96A) | 0.001 | 0.000 | 0.001 |
| Average (miR-16-5p, miR-191-5p, U6 snRNA) | 0.000 | 0.000 | 0.000 |
| Average (SNORD96A, miR-191-5p, U6 snRNA) | 0.000 | 0.000 | 0.000 |
| Average (miR-191-5p,miR-484, SNORD96A) | 0.000 | 0.000 | 0.000 |
| Average (miR-191-5p,miR-484, U6 snRNA) | 0.000 | 0.000 | 0.000 |
| Average (miR-484, SNORD96A,U6 snRNA) | 0.000 | 0.000 | 0.001 |
| Intergroup variation | |||
| Group identifier | OPMD | Controls | Oral cancer |
| miR-16-5p | 0.022 | 0.004 | -0.026 |
| miR-191-5p | 0.009 | -0.001 | -0.007 |
| miR-484 | -0.011 | -0.001 | 0.013 |
| SNORD96A | 0.013 | 0.000 | -0.013 |
| U6 snRNA | -0.027 | 0.001 | 0.027 |
| Average (miR-16-5p, miR-191-5p, miR-484) | 0.005 | 0.000 | -0.005 |
| Average (miR-16-5p, miR-191-5p, SNORD96A) | 0.014 | 0.001 | -0.015 |
| Average (miR-16-5p, miR-191-5p, U6 snRNA) | -0.002 | 0.001 | 0.001 |
| Average (SNORD96A, miR-191-5p, U6 snRNA) | -0.004 | 0.000 | 0.004 |
| Average (miR-191-5p,miR-484, SNORD96A) | 0.003 | -0.001 | -0.002 |
| Average (miR-191-5p,miR-484, U6 snRNA) | -0.012 | -0.001 | 0.012 |
| Average (miR-484, SNORD96A,U6 snRNA) | -0.010 | -0.001 | 0.010 |
| Best gene | Average (miR-191-5p,miR-484, SNORD96A) | ||
| Stability value | 0.004 | ||
The most stable reference gene/combination has the least stability value which is calculated based on the inter-group and intra-group variations
Italicized values: the Normfinder software does not report the level of significance for the stability values. So we are unable to report the same