Table 4.

Fold changes in gene expression under hyphae-inducing condition (RPMI-1640 medium, incubation at 37 °C for 6 h) using the comparative Ct method (ΔΔ Ct).

Gene	Untreated Candida albicans	Fluconazole (2 µg/mL)	Vitamin D3
			6 μg/mL	60 μg/ml
ALS1	0.98	0.56	2.61**	11.96**
SAP6	1.0	0.60	3.17**	3.27**
HWP1	1.0	0.15	0.93	1.20
EFG1	1.0	0.46	12.48**	11.21**

^*P-value ≤ 0.001: P-values were obtained after the comparison of the test results with untreated C. albicans.

Transcription of genes was quantified by reverse transcription-quantitative polymerase chain reaction. Samples were treated with Vitamin D₃ under hyphae-inducing conditions for RNA extraction. Analysis was carried out by analysis of variance. Fold change was calculated by PCR product of the gene of interest/the PCR product of ACT1 (the housekeeping gene) and normalized to the negative control of untreated C. albicans where the expression was considered equal to 1.