FIG. 1.

Western blots with primary goat anti-His-Int₂₈₆ and secondary rabbit anti-goat horseradish peroxidase-conjugated antibody. Lane 1, purified EPEC His-Int₂₈₆; lane 2, purified, histidine-tagged C-terminal third 281 amino acids of intimin γ; lane 3, Δeae EHEC strain 86-24Δ10; lane 4, wild-type strain 86-24; lane 5, wild-type E2348/69 strain; lane 6, Δeae CVD206 strain. Arrows indicate the reaction with full-length intimin α or intimin γ (*) from lysates of wild-type EPEC and EHEC strains, respectively, and the reaction with the purified C-terminal fragment of intimin α or intimin γ (**). Intimin was not detected in lysates of Δeae strains CVD206 or 86-24Δ10, as expected (lanes 3 and 6).