FIG. 2.

Inhibition of binding of EPEC strains to HEp-2 cells in the presence of anti-His-Int₂₈₆. Shown are wild-type strain E2348/69 treated with preimmune serum (A) and anti-His-Int₂₈₆ (B), plasmid-cured strain JPN15 treated with preimmune serum (C) and anti-His-Int₂₈₆ (D), and intimin mutant strain CVD206 treated with preimmune serum (E) and anti-His-Int₂₈₆ (F). The binding of the plasmid-cured strain was greatly reduced following the preincubation of bacteria with anti-intimin antibody as observed by light microscopy.