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. Author manuscript; available in PMC: 2024 Feb 18.
Published in final edited form as: Science. 2023 Jun 16;380(6650):eade0027. doi: 10.1126/science.ade0027

Fig. 5. Slc22a3 regulates histone serotonylation in OB astrocytes.

Fig. 5.

(A) Immunostaining of H3-5HT in OBs of Aldh1l1-GFP mouse and quantification of GFP+/H3-5HT+ co-labeling (n= 3, 25-45 cells). (B) Schematic illustrating viral vectors and timelines for H3-5HT quantification and ChIP-Seq. (C-D) H3-5HT immunostaining and quantification in control and Slc22a3-cKO OB astrocytes (74-79 cells/cohort, *p= 0.0377; unpaired Student’s two-tailed t-test on n= 4 mice/cohort). Scale bar: 5 µm. (E) Venn diagram depicting number of H3-5HT ChIP-Seq peaks unique and shared between control and Slc22a3-cKO OB’s (n=4 OBs/cohort). (F) Venn diagram depicting number of genes that both lose H3-5HT peaks and are downregulated in Slc22a3-cKO. (G) Heatmaps comparing ChIP H3-5HT at 4 kb from peak center in control vs Slc22a3-cKO OB’s. (H) GO analysis of genes at H3-5HT peaks revealing loss of H3-5HT regulation at GABA-associated pathways in Slc22a3-cKO, and (I) of the 538 overlapping genes shown in Figure 5F. See Table S3 for data summary.