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Immunization with V peptides encoding CD4 T-cell epitopes primes antigen-specific CD4 T-cell responses. C57BL/6 mice were immunized with 10 μg of F1-V, 200 μg of OVA, 100 μg of peptide V1, 100 μg of peptide V2, 100 μg of peptide V3, or 100 μg of a control peptide (Vneg) emulsified in CFA. Six days later, CD4 T-cell priming was assessed by measuring in vitro production of IL-2, as in Fig. 1. Data are representative of results obtained in three independent experiments.
