Table 2.
Sequences of oligonucleotide primers1 for real-time quantitative fluorescence PCR.
| Gene | Sequences of primers (5′ to 3′)2 | Serial number |
|---|---|---|
| CLDN1 | F: CATACTCCTGGGTCTGGTTGGT R: GACAGCCATCCGCATCTTCT |
NM_001013611.2 |
| β-Actin | F: CAACACAGTGCTGTCTGGTGGTAC | NM_205518.1 |
| R: CTCCTGCTTGCTGATCCACATCTG | ||
| OCLN | F: ACGGCAGCACCTACCTCAA R: GGGCGAAGAAGCAGATGAG |
NM_205128.1 |
| ZO-1 | F: CTTCAGGTGTTTCTCTTCCTCCTC R: CTGTGGTTTCATGGCTGGATC |
XM_015278981.1 |
| MUC-2 | F: TTCATGATGCCTGCTCTTGTG R: CCTGAGCCTTGGTACATTCTTGT |
XM_421035 |
| IL-1β | F: ACTGGGCATCAAGGGCTA R: GGTAGAAGATGAAGCGGGTC |
NM_204524.1 |
| IL-4 | F: GCTCTCAGTGCCGCTGATG R: GAAACCTCTCCCTGGATGTCAT |
NM-0010079.1 |
| IL-6 | F: CGCCCAGAAATCCCTCCTC R: AGGCACTGAAACTCCTGGTC |
XM_015281283.1 |
| IL-8 | F: ATGAACGGCAAGCTTGGAGCTG | XM_015301388.1 |
| R: TCCAAGCACACCTCTCTTCCATCC | ||
| IL-10 | F: GCTGCCAAGCCCTGTT R: CCTCAAACTTCACCCTCA |
NM_001004414.2 |
| IL-18 | F: TGATGAGCTGGAATGCGATG | NM_204608.3 |
| R: ACTGCCAGATTTCACCTCCTG | ||
| TGF-β3 | F: CATCGAGCTCTTCCAGATCC | NM_205454.1 |
| R: GACATCGAAGGACAGCCACT | ||
| TNF-α | F: GAGCGTTGACTTGGCTGTC R: AAGCAACAACCAGCTATGCAC |
XM_204267 |
| IFN-γ | F: AGCTGACGGTGGACCTATTATT R: GGCTTTGCGCTGGATTC |
NM_205149.1 |
| NF-κB | F: GTGTGAAGAAACGGGAACTG R: GGCACGGTTGTCATAGATGG |
NM_205129.1 |
CLDN1 = claudin-1; OCLN = occludin; ZO-1 = zonula occludens-1; MUC-2 = mucin-2; IL-1β = interleukin-1β; IL-4 = interleukin-4; IL-6 = interleukin-6; IL-8 = interleukin-8; IL-10 = interleukin-10; IL-18 = interleukin-18; TGF-β3 = transforming growth factor-β3; TNF-α = tumor necrosis factor α; IFN-γ = interferon γ; NF-κB = nuclear factor kappa B.
1
Primers were designed using Primer Express software (Sangon Biotech Co., Ltd., Shanghai, China).
2
F for forward; R for reverse.