Table 2.
Analyses of flavonoids and phenolic acids in EtOAc layer by UPLC-ESI-QTOF-MS.
| Peak No. |
tR (min) | Molecular formula | Experiment | MS/MS fragmentation | Identification | References |
|---|---|---|---|---|---|---|
| 1 | 4.06 | C33H40O20 | 755.132 2 | 609, 463, 301, 300, 271, 255 | Quercetin 3-O-glucoside-dirhamnosyl | |
| 2 | 4.3 | C27H30O17 | 625.078 6 | 316, 271 |
Myricetin 3-O-neohesperidoside | |
| 3 | 4.84 | C7H6O5 | 168.988 1 | 126 | Gallic acid | (Vieira, Marques, Machado, Sliva, & Hubinger, 2017) |
| 4 | 5.33 | C28H32O15 | 609.084 0 | 462, 315, 300, 284, 271 |
Isorhamnetin-3-O-dirhamnosyl | |
| 5 | 5.48 | C27H30O15 | 593.089 4 | 284, 255, 227 | Kaempferol-3-O-deoxyhexose-hexose | |
| 6 | 5.77 | C27H30O15 | 755.132 2 | 593, 300, 271, 255 | Quercetin-3-O-dirhamnosyl-glucoside | (Tkacz et al., 2020) |
| 7 | 5.95 | C21H20O10 | 431.143 6 | 284, 255, 285, 271, 227 | Kaempferol-3-O-rhamnoside | (Khallouki, Ricarte, Breuer, & Owen, 2018) |
| 8 | 6.22 | C27H30O17 | 625.078 6 | 316, 287, 271 | Myricetin-3-O-rutinoside | (Wu et al., 2016) |
| 9 | 6.62 | C33H40O19 | 739.137 6 | 593, 284, 255, 151 | Kaempferol-3-O-rutinoside-7-O-rhamnoside | |
| 10 | 6.83 | C21H20O13 | 478.874 4 | 316, 317, 287, 271, 243 | Myricetin 3-O-glucoside | (Zheng et al., 2019) |
| 11 | 6.94 | C27H30O16 | 609.084 | 301, 300, 271, 255, 227 |
Quercetin 7-O-rutinoside | (Cao, Xia, Chen, Xiao, &Wang, 2013) |
| 12 | 7.48 | C27H30O16 | 609.078 | 301, 300, 271, 255 | Quercetin 3-O-rutinoside | (Zheng et al., 2019) |
| 13 | 7.84 | C42H46O22 | 900.940 4 |
755, 609, 447, 301, 300 |
Quercetin-7-O-dirhamnosyl-diglucoside | |
| 14 | 8.05 | C27H30O15 | 593.092 6 | 284, 285, 255, 227 | Kaempferol 3-O-neohesperidoside | (Wang et al., 2014) |
| 15 | 8.22 | C21H20O12 | 463.037 8 | 317, 316, 271, 259 | Myricitrin | (Unuofin & Lebelo, 2021) |
| 16 | 8.48 | C21H20O12 | 463.0378 | 301, 300, 271, 255, 243, 227 | Isoquercitrin | (Braunberger et al., 2013) |
| 17 | 8.93 | C27H30O15 | 593.092 6 | 285, 255, 227 | Kaempferol-3-O-rutinoside | Vieira, Marques, Machado, Silva, & Hubinger, 2017 |
| 18 | 9.26 | C28H32O16 | 623.098 | 463, 315, 300, 271, 255 | Isorhamnetin 3-O-rutinoside |
Tkacz et al., 2020 |
| 19 | 9.63 | C26H28O16 | 554.203 1 | 300, 284, 271, 255, 227 | Kaempferol-7-O-hexosyl(1–2)deoxyhexoside | |
| 20 | 10.06 | C21H20O11 | 447.043 7 | 284, 255, 227 | Kaempferol 3-O-glucoside | Vieira, Marques, Machado, Silva, & Hubinger, 2017 |
| 21 | 10.35 | C21H20O11 | 447.043 7 | 301, 300, 271, 255, 243, 227 | Quercitrin | Hui et al., 2017 |
| 22 | 11.60 | C22H22O11 | 460.903 0 | 299, 297, 284, 269, 255 | Chrysoeriol 7-O-glucoside |
Zheng et al., 2019 |
| 23 | 12.17 | C22H22O12 | 476.894 5 | 390, 315, 300, 272, 255 | Isorhamnetin-3-O-glucoside |
Khallouki, Ricarte, Breuer, & Owen, 2018 |
| 24 | 12.45 | C21H20O10 | 430.901 3 | 285, 255, 227 |
Kaempferol 7-O-rhamnoside | Hui et al., 2017 |