. 2024 Feb 12;9(1):371–385. doi: 10.1089/can.2022.0216

Table 1.

Mass Spectrometry Parameters of the Method

Analyte	Retention time (minutes)	Precursor ion (m/z)	Product ion (m/z)	Fragmentation voltage (V)	Collision energy (V)
[²H₃]-11-OH-THC^a	2.1	334.20	316.1	140	10
[²H₃]-11-OH-THC^b	2.1	334.20	105.1	140	50
11-OH-THC^a,c	2.1	331.23	313.1	133	9
11-OH-THC^b	2.1	331.23	105.0	133	45
[²H₃]-11-COOH-THC	2.4	348.20	330.2	144	13
11-COOH-THC^a,c	2.4	345.20	327.2	142	13
11-COOH-THC^b	2.4	345.20	299.2	142	17
[²H₃]-CBD^a	3.4	318.20	196.2	150	22
[²H₃]-CBD^b	3.4	318.20	123.0	150	38
CBD^a	3.4	315.20	193.1	138	21
CBD^b	3.4	315.20	123.0	138	37
[²H₃]-THC^a	6.1	318.20	196.1	145	10
[²H₃]-THC^b	6.1	318.20	93.1	145	22
THC^a,c	6.1	315.20	193.1	147	21
THC^b	6.1	315.20	123.0	147	37
[²H₄]-Anandamide^a,c	7.0	352.32	66.2	140	50
[²H₄]-Anandamide^b	7.0	352.32	67.1	140	14
Anandamide^a	7.0	348.29	62.2	128	13
Anandamide^b	7.0	348.29	91.1	128	45
[²H₅]-2-AG^a,c	7.6	384.30	287.2	125	10
[²H₅]-2-AG^b	7.6	384.30	67.1	125	50
2-AG^a	7.6	379.29	287.2	123	10
2-AG^b	7.6	379.29	269.2	123	13
VEA^a	10.0	326.30	62.1	120	14
VEA^b	10.0	326.30	55.1	133	42
[²H₄]-OEA^a,c	10.3	330.34	66.1	140	14
[²H₄]-OEA^b	10.3	330.34	312.8	140	22
OEA^a	10.3	326.31	62.1	133	13
OEA^b	10.3	326.31	55.1	133	45
[²H₄]-PEA^a,c	12.6	304.32	66.2	125	14
[²H₄]-PEA^b	12.6	304.32	287.2	125	18
PEA^a	12.6	300.29	62.2	128	13
PEA^b	12.6	300.29	57.1	128	33

^{^a}

Quantifier.

^{^b}

Qualifier.

^{^c}

Used in matrix effect studies.

11-COOH-THC, 11-nor-9-carboxy-Δ⁹-THC; 11-OH-THC, 11-hydroxy-Δ⁹-THC; 2-AG, 2-arachidonoyl-sn-glycerol; CBD, cannabidiol; OEA, oleoylethanolamide; PEA, palmitoylethanolamide; THC, Δ⁹-tetrahydrocannabinol; VEA, vaccenoylethanolamide.