Table 4.
Interday Accuracy and Precision of Analyte Quantification in Pre-Enhanced Matrix Removal Spiked Plasma
| Measured amount (ng/mL) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Nominal quantity (ng/mL) | Analyte | Day 1 |
Day 2 |
Day 3 |
||||||
| Mean | %RSD | Accuracy (%) | Mean | %RSD | Accuracy (%) | Mean | %RSD | Accuracy (%) | ||
| 5 | OH-THC | 4.24 | 1.55 | 84.88 | 4.69 | 2.61 | 93.86 | 4.39 | 1.78 | 87.74 |
| COOH-THC | 4.48 | 2.10 | 89.62 | 4.27 | 1.92 | 85.39 | 4.42 | 2.40 | 88.44 | |
| CBD | 4.60 | 0.11 | 92.06 | 4.46 | 1.79 | 89.19 | 4.45 | 2.34 | 89.04 | |
| THC | 4.34 | 1.16 | 86.84 | 4.38 | 2.13 | 89.52 | 4.33 | 0.09 | 86.55 | |
| Anandamide | 5.37 | 2.83 | 97.68 | 5.39 | 1.32 | 97.79 | 5.46 | 2.41 | 99.28 | |
| 2-AG | 13.61 | 4.39 | 108.89 | 14.56 | 8.90 | 96.10 | 16.03 | 6.01 | 105.15 | |
| VEA | 6.23 | 1.08 | 102.60 | 6.47 | 2.46 | 106.91 | 6.18 | 1.90 | 101.69 | |
| OEA | 6.34 | 0.79 | 96.09 | 6.29 | 4.04 | 95.76 | 6.33 | 0.07 | 96.07 | |
| PEA | 9.90 | 13.14 | 107.25 | 9.89 | 8.94 | 108.89 | 10.06 | 10.85 | 108.14 | |
| 50 | OH-THC | 50.92 | 3.04 | 101.84 | 56.89 | 1.64 | 113.79 | 51.18 | 3.06 | 102.35 |
| COOH-THC | 50.84 | 4.25 | 101.68 | 49.98 | 2.12 | 99.96 | 50.96 | 2.01 | 101.92 | |
| CBD | 50.99 | 4.36 | 101.98 | 50.75 | 1.09 | 101.49 | 51.90 | 1.36 | 103.81 | |
| THC | 50.64 | 3.35 | 101.27 | 53.83 | 3.91 | 107.66 | 51.28 | 1.92 | 102.56 | |
| Anandamide | 50.65 | 2.35 | 100.30 | 50.55 | 2.34 | 100.07 | 50.14 | 1.98 | 99.27 | |
| 2-AG | 58.13 | 6.47 | 101.11 | 57.81 | 8.11 | 96.11 | 55.00 | 4.91 | 91.29 | |
| VEA | 52.92 | 1.05 | 86.19 | 55.71 | 7.41 | 109.12 | 51.67 | 2.11 | 101.18 | |
| OEA | 50.64 | 3.35 | 98.13 | 53.83 | 3.91 | 104.39 | 51.28 | 1.95 | 99.41 | |
| PEA | 52.21 | 2.63 | 96.28 | 48.70 | 2.77 | 90.05 | 50.93 | 3.73 | 93.78 | |
| 200 | OH-THC | 205.27 | 0.97 | 102.63 | 228.44 | 1.43 | 114.22 | 210.33 | 1.43 | 105.17 |
| COOH-THC | 208.68 | 1.07 | 104.35 | 204.64 | 1.14 | 102.33 | 216.06 | 0.86 | 107.86 | |
| CBD | 211.56 | 0.77 | 105.78 | 205.55 | 1.20 | 102.77 | 211.28 | 0.37 | 105.64 | |
| THC | 213.17 | 0.56 | 106.59 | 220.48 | 1.98 | 110.24 | 207.81 | 3.94 | 103.90 | |
| Anandamide | 228.14 | 1.45 | 113.78 | 227.87 | 0.98 | 113.78 | 225.68 | 0.86 | 112.56 | |
| 2-AG | 247.13 | 0.73 | 119.10 | 241.70 | 2.27 | 115.01 | 235.65 | 1.19 | 112.08 | |
| VEA | 203.30 | 0.86 | 101.11 | 198.99 | 0.78 | 98.97 | 194.01 | 0.17 | 96.49 | |
| OEA | 227.13 | 0.79 | 112.66 | 221.70 | 2.48 | 109.99 | 215.65 | 1.29 | 106.98 | |
| PEA | 231.97 | 1.98 | 113.59 | 223.25 | 8.09 | 109.39 | 231.56 | 4.36 | 113.34 | |
Analyses were run on 3 consecutive days. The standard error of the mean was in all cases ≤20% and was omitted for clarity.
%RSD, percent relative standard deviation; EMR, Enhanced Matrix Removal.