Table 5.
Analyte Recovery Following Enhanced Matrix Removal Fractionation from Plasma or Brain Tissue
| |
Plasma |
Brain |
|
|---|---|---|---|
| [ng/mL] | Analyte | Recovery (%) | Recovery (%) |
| 5 | OH-THC | 95.2 | 91.9 |
| COOH-THC | 96.9 | 98.4 | |
| CBD | 88.5 | 92.2 | |
| THC | 87.7 | 90.4 | |
| Anandamide | 93.7 | 88.5 | |
| 2-AG | 94.3 | 94.3 | |
| VEA | 98.9 | 90.5 | |
| OEA | 101.6 | 88.2 | |
| PEA | 101.6 | 86.2 | |
| 50 | OH-THC | 88.4 | 102.0 |
| COOH-THC | 90.8 | 99.0 | |
| CBD | 86.0 | 98.9 | |
| THC | 86.7 | 102.6 | |
| Anandamide | 87.3 | 104.2 | |
| 2-AG | 100.8 | 100.8 | |
| VEA | 86.2 | 100.9 | |
| OEA | 94.6 | 99.1 | |
| PEA | 85.6 | 95.9 | |
| 200 | OH-THC | 101.5 | 92.1 |
| COOH-THC | 93.6 | 93.5 | |
| CBD | 102.2 | 91.8 | |
| THC | 101.0 | 94.9 | |
| Anandamide | 91.3 | 93.6 | |
| 2-AG | 93.9 | 94.0 | |
| VEA | 86.3 | 99.8 | |
| OEA | 104.0 | 90.1 | |
| PEA | 104.0 | 100.5 | |
Recovery (%) was calculated as (Response pre-EMR/Response post-EMR)×100, where response pre-EMR is the average area for the analytes, which have gone through the fractionation process. Response post-EMR is the average area for the same quantity of analyte spiked into the extracted matrix after the fractionation procedure (n=3/condition, run in duplicate).