Figure 1.
GATA6 and SOX2 expression report on an ICM-like sub-population in certain culture states for naive pluripotency
(A) Schematic drawing of the SGGC double reporter.
(B) Flow cytometry contour plots of the SGGC cell lines in the stated media conditions. SL, serum/LIF.
(C) Immunofluorescence images of SOX2 and GATA6 in the stated conditions. Scale bar, 30 μm, yellow stars indicate co-expression.
(D) UMAP of scRNA-seq dataset where coloring represents Louvain clustering.
(E) UMAP of scRNA-seq dataset showing the different cell populations sequenced.
(F) Heatmap showing normalized residuals post-χ2 test comparing differences in proportions of the Seurat clusters mapped to in vivo scRNA-seq of pre-implantation blastocysts (Nowotschin et al., 2019). Color scale represents the normalized deviation of observed from expected proportions.
(G) Dot plot graph showing the log normalized average expression of selected genes across different media conditions. The average expression is marked by the color scale and the percentage of expression by the size of the circle.