FIGURE 1.

Treatment with LY344864 increases FCCP-OCR levels, phosphorylation of AKT, and mitochondrial number and returns to baseline after 24 h of removal in RPTC. (A) FCCP-OCR levels analysis. (B) Densitometry analysis of PGC-1α. (C) Densitometry analysis of DRP1. (D) Densitometry analysis of p-AKT and AKT. (E) Representative immunoblots. (F) Representative electron micrographs. (G) Mitochondrial number. (H) Mitochondrial area (I) Mitochondrial length. Data represents n = 4–6 and are expressed as mean ± SEM; p < 0.05 compared by a one-way ANOVA (followed by a Dunnet’s post-hoc test). Different letters on top of bars represent different statistical significance. Scale bars represent 500 nm.