Skip to main content
. 2005 Apr 1;4:9. doi: 10.1186/1475-2859-4-9

Table 3.

Primers for synthesis of in vitro RNA standards. The T7 promoter sequence was added to the 5' side of all primer 1 sequences.

Name Sequence (5'-3') Position
dnaK
Primer 1 CTCTTGTGTAGCGATTATGG 39
Primer 2 GTTGTTTGCAGAAGCATCAG 1860c
Ibp
Primer 1 CCGCTTTACCGTTCTGCTA 22 (ibpA)
Primer 2 CTATTTAACGCGGGACGTTC 425c (ibpB)
Lon
Primer 1 ATGAATCCTGAGCGTTCTGA 1
Primer 2 CACAACCTGCATACCAGAC 2342c
T7 promoter CTAATACGACTCACTATAGGGAGA