Fig. 5.

Comparison of NF and t3 hyperthermia regimen on Monocyte to M1 macrophage differentiation. Phenotype analysis of PBMC differentiated into M1 macrophages in the presence of PLGA scaffolds at different concentration of GO (0.5, 1, 2 and 5%) in comparison to the control condition in red, second lane, represented by fully differentiated M1 macrophages treated with the same hyperthermia regimen, at the two different hyperthermia regimens: NF (A, upper panel) or t3 (B, lower panel). Scaffolds were added at the start of the differentiation protocol to evaluate the ability to affect monocyte differentiation toward M1 macrophages. At the end of the culture period, expression of CD14, CD209, CD197 and CD163 and of the co-stimulatory molecule CD80 and CD86 was evaluated by flow cytometry. Results are presented as a percentage of expression and are shown violin plots show median (thick line), 25th and 75th quartiles (*p < 0.05, ***p < 0.001, ****p < 0.0001 versus control M1), N ≥ 3 individual experiments.