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Immunoblot detection with Strep Tactin HPR conjugate (IBA GmbH, Göttingen, Germany) of Strep tag II-HydACa in sonicated C. acetobutylicum cells with Strep tag II in the N-terminal position (A) (lane 1, postsonication pellet; lane 2, cell extract) and Strep tag II in the C-terminal position (B) (lane 1, cell extract; lane 2, postsonication pellet). M, prestained low-range standard proteins (sizes are in kDa).
