Figure 5.

Effects of small side chain substitutions of W207 on the closed state of the channel.A, representative tail currents recorded from WT (207W), 207S, 207A, and 207G. The tail currents were elicited by a prepulse to 120 mV, in 10 mV decrements from 0 to −60 mV. Red arrows indicate faster deactivation of W207 mutations. B, the deactivation (channel closing) time constant Ʈ_deact in WT or W207 mutations. Ʈ_deact was obtained from exponential fit to the tail currents, n = 3 to 6 for each group. Ʈ_deact between W207 mutation and WT were compared statistically using two-tailed test (∗∗∗p < 0.001).