TABLE 2.

Primers used for amplification of transcripts by real-time RT-PCR

Primer name	Primer sequence (5′-3′)a	Primer %GC	Amplicon size (bp)	Amplicon Tmb
				expected	observed
F/2Contig26	For: CACCATCAATACATCGTCGGAT	45	82	78	81.5
	Rev: CTGGATCATAATCACGCACGAA	45
F/2Contig34	For: TTTGTCGAACTTCCGCATGA	45	78	83	85.5
	Rev: TCGTTGGCGCACATACTGA	53
F/2Contig22	For: AGGCCATCTACAACCACTACAAACC	48	77	81	83
	Rev: CCTCATGCAATTCGTGTTCCA	48
F/50Contig29	For: AGTTGAAGTGCTGCCCTGGTT	52	97	82	85.2
	Rev: TCGTAGACAGTCCGTGGTTCCT	55
F/50Contig31	For: CCTAGGACAGTTCATCCAAACGA	48	96	79	83.5
	Rev: GCAAAGCACAAGGACTCATCAA	45
F/50Contig18	For: GACTTCGCCAGCTACAATCCA	52	84	81	84
	Rev: GGCGAACTTTGCCATATCCTT	48
F/50Contig36	For: GTGTCACGCCCTCTCAATCAGT	55	76	83	85
	Rev: TGTTCGCCAGAATCGTTTCAC	48
F/50Contig33	For: CCATCCTGCAGTCCTTGATACC	55	84	82	85.5
	Rev: AGATGCCGTTTGCGCTTT	50

^aFor, forward; Rev, reverse.

^bT_m, melting temperature.