TABLE 4.
BLASTX results of most prevalent transcripts from f/2-specific SSH librarya
| SeqID | Putative ID | e value | Reads | Length |
|---|---|---|---|---|
| Sig1 | Unknown | >0.001 | 1 | 865 |
| Sig2 | ORF497, homologous to Porphyra ORF491 (Odontella sinensis) | 2.47E-28 | 1 | 797 |
| Sig3 | Unknown | >0.001 | 1 | 828 |
| Sig4 | Unknown | >0.001 | 1 | 543 |
| Sig5 | Unknown | >0.001 | 1 | 479 |
| Sig6 | Apocytochrome f, chloroplast precursor | 7.499E-13 | 1 | 866 |
| Sig7 | RNA polymerase beta apos; apos; subunit (Cyanidium caldarium) | 3.593E-09 | 1 | 628 |
| Sig8 | Pentose-5-phosphate-3-epimerase (Pseudomonas fluorescens PfO-1) | 6.42E-16 | 1 | 804 |
| Con2 | Ribosomal protein S9 (Crocosphaera watsonii WH 8501) | 1.36E-29 | 1 | 960 |
| Con3 | RNA polymerase beta prime subunit (Gloeobacter violaceus PCC 7421) | 4.26E-38 | 1 | 913 |
| Con4 | Ribosomal protein S3 (Nostoc punctiforme) | 1.09E-31 | 1 | 803 |
| Con5 | Ribosomal protein S3 (Porphyra purpurea) | 3.29E-28 | 1 | 807 |
| Con6 | Ribosomal protein S3 (Porphyra purpurea) | 1.72E-16 | 1 | 916 |
| Con7 | Unknown | >0.001 | 1 | 801 |
| Con8 | Orf122-(Chlorobium tepidum) | 5.686E-10 | 1 | 1,002 |
| Con9 | Unknown | >0.001 | 2 | 322 |
| Con10 | Hypothetical protein (Trichodesmium erythraeum IMS101) | 3.08E-40 | 2 | 634 |
| Con11 | Unknown | >0.001 | 2 | 433 |
| Con12 | Unknown | >0.001 | 2 | 416 |
| Con14 | Ribosomal protein S2 (Guillardia theta) | 4.21E-24 | 2 | 863 |
| Con15 | Unknown | >0.001 | 2 | 1,174 |
| Con16 | Ribosomal protein S4 (Nephroselmis olivacea) | 5.61E-26 | 2 | 447 |
| Con17 | Unknown | >0.001 | 2 | 419 |
| Con18 | Hypothetical protein (Bacillus megaterium) | 8.79E-17 | 2 | 396 |
| Con19 | Unknown | >0.001 | 2 | 392 |
| Con20 | Unknown | >0.001 | 2 | 405 |
| Con21 | Unknown | >0.001 | 2 | 645 |
| Con22 | Unknown | >0.001 | 2 | 455 |
| Con23 | Signal transduction histidine kinase (Crocosphaera watsonii WH 8501) | 1.93E-30 | 2 | 781 |
| Con24 | Unknown | >0.001 | 2 | 301 |
| Con25 | Elongation factor Tu GTP binding domain (Bacillus anthracis A2012) | 3.32E-33 | 2 | 897 |
| Con26 | Photosystem I P700 chlorophyll a apoprotein A2 (Emiliania huxleyi) | 1.76E-74 | 2 | 616 |
| Con27 | Hypothetical protein (Plasmodium yoelii yoelii) | 5.41E-19 | 3 | 961 |
| Con28 | ATP synthase delta chain, chloroplast | 1.37E-30 | 3 | 572 |
| Con29 | Unknown | >0.001 | 3 | 402 |
| Con30 | ABC transporter (Cyanophora paradoxa) | 2.18E-49 | 3 | 621 |
| Con31 | Ribosomal protein S9 (Crocosphaera watsonii WH 8501) | 1.18E-32 | 3 | 587 |
| Con32 | Hypothetical protein (Nostoc sp. strain PCC 7120) | 3.50E-18 | 3 | 869 |
| Con33 | 50S ribosomal protein L3 (Prochlorococcus marinus) | 1.08E-19 | 3 | 537 |
| Con34 | Unknown | >0.001 | 4 | 1,021 |
| Con35 | Hypothetical chloroplast RF7 (Guillardia theta) | 6.85E-04 | 5 | 364 |
| Con36 | Hypothetical protein Avar020180 (Anabaena variabilis ATCC 29413) | 0.0001666 | 5 | 514 |
| Con37 | Preprotein translocase subunit Sec Y (Porphyra purpurea) | 8.27E-20 | 6 | 866 |
| Con38 | COG0087: ribosomal protein L3 (Trichodesmium erythraeum IMS101) | 1.79E-11 | 7 | 465 |
| Con39 | Chloroplast ATP synthase a chain precursor (ATPase subunit IV) | 1.54E-76 | 7 | 745 |
| Con40 | Subunit epsilon of ATPase (Ochrosphaera neapolitana) | 8.68E-30 | 7 | 1,020 |
| Con41 | Photosystem 1 subunit III (Guillardia theta) | 1.11E-39 | 8 | 1,027 |
| Con42 | Photosystem 1 subunit XI (Guillardia theta) | 2.82E-35 | 8 | 1,152 |
| Con43 | Predicted protein (Methanosarcina acetivorans strain C2A) | 1.02895 | 10 | 619 |
| Con44 | Orf122 (Chlorobium tepidum) | 8.41E-19 | 10 | 971 |
| Con45 | Ribosomal protein S2 (Guillardia theta) | 1.41E-56 | 10 | 566 |
| Con46 | Hypothetical protein TC0128 (imported)—Chlamydia muridarum (strain Nigg) | 0.116837 | 12 | 692 |
| Con47 | COG0092: ribosomal protein S3 (Nostoc punctiforme) | 6.21E-31 | 19 | 651 |
| Con48 | Hypothetical protein (Chlamydophila pneumoniae AR39) | 2.60643 | 56 | 1,570 |
| Con49 | COG0092: ribosomal protein S3 (Anabaena variabilis ATCC 29413) | 1.27E-40 | 71 | 1,128 |
| Con50 | COG0048: ribosomal protein S12 (Crocosphaera watsonii WH 8501) | 1.81E-37 | 151 | 1,766 |
a
BLASTX results following PHRAP assembly are listed as follows: SeqID (sequence identification number) for singletons (Sig) and contigs (Con), Reads (numbers of EST fragments per assembled sequence), and Length (average sequence length in base pairs).