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. 2024 Feb 5;14(3):e4931. doi: 10.21769/BioProtoc.4931

Table 1. DNA quantities (Challis et al., 2019).

Plasmid For 1 mL of cells For 90 mL of cells
Helper plasmid (AdΔF6) 0.57 µg 51.3 µg
AAV Rep/Cap plasmid 1.13 µg 101.7 µg
AAV shuttle vector 0.30 µg 27 µg
Total DNA 2 µg 180 µg