Table 1. DNA quantities (Challis et al., 2019).
Plasmid | For 1 mL of cells | For 90 mL of cells |
---|---|---|
Helper plasmid (AdΔF6) | 0.57 µg | 51.3 µg |
AAV Rep/Cap plasmid | 1.13 µg | 101.7 µg |
AAV shuttle vector | 0.30 µg | 27 µg |
Total DNA | 2 µg | 180 µg |
Plasmid | For 1 mL of cells | For 90 mL of cells |
---|---|---|
Helper plasmid (AdΔF6) | 0.57 µg | 51.3 µg |
AAV Rep/Cap plasmid | 1.13 µg | 101.7 µg |
AAV shuttle vector | 0.30 µg | 27 µg |
Total DNA | 2 µg | 180 µg |