Table 2. OptiPrep gradient components.

% OptiPrep	60% OptiPrep	Buffer	Phenol red
15%	4 mL	12 mL PBS-NMK	n/a
25%	6.7 mL	9.3 mL PBS-MK	40 µL
40%	10 mL	5 mL PBS-MK	n/a
60%	10 mL	n/a	25 µL