Reagent	Final concentration	Quantity
1 M Tris-HCl pH 7.6	20 mM	2 mL
NaCl	50 mM	0.2922 g
Glycerol	50% (v/v)	50 mL
1 M DTT	1 mM	100 µL
DNase I	10 mg/mL	1 g
ddH2O	n/a	Add up to 100 mL
Total	n/a	100 mL