Reagent	Final concentration	Quantity
1 M Tris-HCl pH 8.0	50 mM	50 mL
2 M MgCl2	5 mM	2.5 mL
NaCl	150 mM	8.77 g
ddH2O	n/a	Add up to 1,000 mL
Total	n/a	1,000 mL