Reagent | Final concentration | Quantity |
---|---|---|
HEPES | 50 mM | 2.980 g |
NaCl | 150 mM | 2.192 g |
Sarcosyl | 1% | 2.5 g |
EDTA | 20 mM | 1.46 g |
ddH2O | n/a | Add up to 250 mL |
Total | n/a | 250 mL |
Reagent | Final concentration | Quantity |
---|---|---|
HEPES | 50 mM | 2.980 g |
NaCl | 150 mM | 2.192 g |
Sarcosyl | 1% | 2.5 g |
EDTA | 20 mM | 1.46 g |
ddH2O | n/a | Add up to 250 mL |
Total | n/a | 250 mL |