TABLE 3.

Optimized primer and probe concentrations for the duplex 5′ nuclease assays

Target	5′ Nuclease assay	Concn (nM) of:
		Forward primer	Reverse primer	Probe
B. adolescentis	B. adolescentis	300	150	100
	All bifidobacteria	300	600	100
B. angulatum	B. angulatum	900	900	200
	All bifidobacteria	300	300	50
B. bifidum	B. bifidum	600	600	200
	All bifidobacteria	300	300	100
B. breve	B. breve	300	300	100
	All bifidobacteria	450	450	150
B. catenulatum	B. catenulatum	300	300	100
	All bifidobacteria	600	600	100
B. dentium	B. dentium	900	900	200
	All bifidobacteria	300	300	50
B. infantis	B. infantis	300	300	100
	All bifidobacteria	900	900	100
B. longum	B. longum	300	300	100
	All bifidobacteria	600	600	200
All bifidobacteria	All bifidobacteria	450	450	100
	All bacteria	900	900	200