FIG. 1.

Schematic representation of primers used for PCR amplifications (A) and comparison of the organizations of genes for the synthesis of trehalose and mannosylglycerate (B) in T. thermophilus strains RQ-1, HB27, Fiji3 A1, PRQ-14, AT-62, B, T-2, CC-16, HB8, and GK24. The mpgS and mpgP sequences of strain HB27; the otsA, otsB, and treS sequences of strain RQ-1; the treS sequence of strain AT-62; and the otsB and treS sequences of strain GK24 were previously described (12, 16, 26, 31). The identities (%) of the deduced amino acid sequences to the corresponding proteins from RQ-1 and HB27 are shown inside the arrows. Unshaded arrows represent the α-glucosidase gene (EC 3.2.1.20).