FIG. 3.

Microphotographs showing cytokine-specific immunoperoxidase staining of cells in cryopreserved antral tissue obtained from H. pylori-infected subjects and uninfected volunteers. The sections were counterstained with hematoxylin. (A) IL-8-specific staining in the antrum of an H. pylori-infected AS carrier (i.e. with gastritis only), showing the typical patchy distribution of epithelial IL-8 reactivity (arrows) and cytoplasmic localization of IL-8 in a monocyte (arrowhead). Original magnification, ×125. (B) Epithelial staining for IL-6 localized to the superficial as well as to the neck and pit epithelium in the antrum of an AS carrier. Original magnification, ×100. (C) Biopsy specimen from an AS carrier showing IL-1β staining of the epithelium as well as of MNCs in the lamina propria. Original magnification, ×200. (D) IL-1β-specific staining of MNCs in the antrum of an uninfected individual. Original magnification, ×160. (E) Intraepithelial IFN-γ-producing lymphocytes in the neck region of a cross-sectioned antral gland in a biopsy sample obtained from an H. pylori-infected DU patient. Original magnification, ×400. (F) Epithelial staining for TGF-β in a biopsy specimen from an uninfected subject. Original magnification, ×125.