TABLE 3.

Deduced functions of all the auxiliary genes identified in the megalomicin gene cluster involved in the biosynthesis of Ery C

Gene	Closest protein match	% Sima	Proposed pathwayb	Proposed function	Reference
megF	EryF	91		C-6 hydroxylase	32
megK	EryK	90		C-12 hydroxylase	25
megL	MtmD	73	Myc/Meg/Des	TDP-d-glucose synthase	16
megM	GilE	73	Myc/Meg/Des	TDP-glucose dehydratase	4
megBVI	EryBVI	86	Myc/Meg	2,3-Dehydratase	27
megBII	EryBII	72	Myc/Meg	2,3-Reductase	27
megDIV	EryBVII	68	Myc/Meg	5-Epimerase	27
megBIII	EryBIII	89	Myc	3-C-Methyltransferase	27
megBIV	EryBIV	80	Myc	4-Ketoreductase	27
megBV	EryBV	85	Myc	Glycosyltransferase	27
megCII	EryCII	78	Des	3,4-Isomerase	27
megCIV	EryCIV	92	Des	2,3-Dehydratase	27
megCV	EryCV	87	Des	3,4-Enoylreductase	6
megDIII	EryCVI	65	Des/Meg	Dimethyltransferase	27
megCIII	EryCIII	90	Des	Glycosyltransferase	27
megDII	Med-ORF20	83	Des/Meg	Aminotransferase	9

^aSim, similarity. Determined by BLASTX using default parameters.

^bMyc, mycarose; Meg, megosamine; Des, desosamine.