PKCμ and Cx43 colocalize in HaCaT cells
(A) Representative images of immunofluorescence staining of PKCμ (green) and Cx43 (red) in HaCaT cells treated with PMA at different time points. Scale bar = 10 μm.
(B) Immunofluorescence images of intact and wounded human skin stained for pPKCμ-S916, total PKCμ, pCx43-S368, and total Cx43. Scale bar = 100 μm.
(C) Relative pPKCμ-S916 levels in intact and wounded skin based on immunofluorescence signal intensity of images shown in (B). Error bars represent S.D. ∗∗∗p < 0.001 (Student’s t test).