Figure 5.

PKCμ regulates wound healing through gap junction mediated intercellular communication

(A) Representative fluorescent microscopic images of SL/DT assay in HaCaT cells treated in the absence or presence of CRT (1 μM CRT), with or without CBX (100 μM) for 6 h, followed by PMA (0.5 μM) for 30 min. Scale bar = 100 μm.

(B) Quantification of dye migration area from three replicate experiments as shown in (A).

(C) Representative images of in vitro wound healing assay in HaCaT cells treated with PMA (10 nM) in the presence or absence of CRT (1 μM), with or without CBX (50 μM) at 0 and 16 h. Scale bar = 100 μm.

(D) Percentage of wound area remaining after 16 h from three replicate experiments in (C).

(E) Quantification of dye migration area from SL/DT assays in HaCaT cells expressing shGFP, shPKCμ A, or shPKCμ B, and treated with or without CBX (100 μM) for 6 h followed by PMA (0.5 μM) for 30 min from three replicate experiments in (Figure S4A).

(F) Percentage of wound area remaining after 16 h in HaCaT cells expressing with shGFP, shPKCμ A, or shPKCμ B, in the absence or presence of PMA (0.5 μM), with or without CBX (100 μM) for 16 h from three replicate experiments in (Figure S4B).

(G) Representative fluorescent microscopic images of SL/DT assay in HaCaT cells expressing shGFP or shCx43 and treated in the absence or presence of CRT (1 μM) for 5 h with or without PMA (0.5 μM) for 30 min. Scale bar = 100 μm.

(H) Quantification of dye migration area from three replicate experiments as shown in (G).

(I) Representative images of in vitro wound healing assay of shGFP or shCx43 treated HaCaT cells treated with PMA (10 nM) in the presence or absence of CRT (1 μM) at 0 and 16 h. Scale bar = 100 μm.

(J) Percentage of wound area remaining after 16 h from three replicate experiments shown in (I). All calculations are based on three replicates −/+ S.D. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test).