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RNA editing in HeLa cells
Representative images from samples of ADAR1 p.110 (ADAR1)-overexpressing and ADAR2-overexpressing HeLa cells transfected with both the TRPM1 nonsense mutation reporter plasmid and 60-mer chemically modified gRNA at 96 h post-seeding. (A) Schematic of the in-vitro experiment. (B) Fluorescent microscopy. (C) Sanger sequence. (D) Next-generation sequencing reads in IGV viewer.
