TABLE 1.
Strains and plasmids used
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| P. fluorescens strains | ||
| F113 | Wild-type strain isolated from sugar beet rhizosphere | 37 |
| F113rif | Rifampin-resistant derivative of F113 | 17 |
| F113 LacZY | F113rif::lacZY | 17 |
| F113pcb | F113rif::bph | 9 |
| F113L::1180 | F113LacZY::nodD-nodbox 4bph | This study |
| Plasmids | ||
| pMP220 | IncP Tetr, promoterless lacZ | 39 |
| pHC60 | IncP Tetr, gfp under the control of a constitutive lac promoter | 10 |
| pMP92 | IncP Tetr | 39 |
| pGFP92 | IncP Tetr, promoterless gfp 65S/T | This study |
| pRK2013 | Helper plasmid, Tra+ Kanr | 18 |
| pDDPCB | pUT derivative, miniTn5::bph, Ampr | 16 |
| pCR2.1-TOPO | PCR cloning vector, Ampr Kanr | Invitrogen |
| pBluescript SK+ | Cloning vector, Ampr | Stratagene |
| pBG1075 | pMP220 nodbox 4::lacZ | This study |
| pBG1079 | pMP220 nodD1::lacZ | This study |
| pBG1106 | pMP220 nodD1-nodbox 4::lacZ | This study |
| pBG1093 | pMP220 nodD1-nodbox 1::lacZ | This study |
| pBG1094 | pMP220 nodbox 1::lacZ | This study |
| pBG1110 | pBG1106 ΔnodD1 | This study |
| pBG1157 | pGFP92 nodD1-nodbox 4::gfp | This study |
| pBG1180 | pDDPCB miniTn5::nodD1-nodbox 4::bph | This study |
| pBG1197 | PMP92 nodD1-nodbox 4::bph | This study |