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Cell cycle analysis of HS-72 cells cultured with the toxin from A. actinomycetemcomitans Y4 by flow cytometry. HS-72 cells (106) were cultured with the partially purified toxin (50 μg/ml) for 48 h and then stained with propidium iodide. DNA content was analyzed at the times indicated. □, G1 phase; 
, S phase; ▨, G2/M phase; ■, apoptosis.
