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Schematic overview of the different PCR-DGGE strategies used to study the diversity of sulfate-reducing bacteria in complex microbial communities of anaerobic bioreactors. (A) One-step direct PCR-DGGE strategy; (B) two-step nested-PCR-DGGE strategy; (C) three-step nested-PCR-DGGE strategy. Comparative DGGE analysis of PCR products obtained by strategies A/B and C made it possible to infer the relative abundance of SRB in the samples analyzed.
