TABLE 1.
Primers used in this study
| Primer set | Target group | Target organism(s) | Positions | Annealing temp | Reference |
|---|---|---|---|---|---|
| DFM140 | 1 | Desulfotomaculum | 140-158 | 58°C | 2 |
| DFM842 | 823-842 | ||||
| DBB121 | 2 | Desulfobulbus | 121-142 | 66°C | 2 |
| DBB1237 | 1215-1237 | ||||
| DBM169 | 3 | Desulfobacterium | 169-183 | 64°C | 2 |
| DBM1006 | 986-1006 | ||||
| DSB127 | 4 | Desulfobacter | 127-148 | 60°C | 2 |
| DSB1273 | 1252-1273 | ||||
| DCC305 | 5 | Desulfonema-Desulfo- sarcina-Desulfococcus | 305-327 | 65°C | 2 |
| DCC1165 | 1144-1165 | ||||
| DSV230 | 6 | Desulfovibrio-Desulfo- microbium | 230-248 | 61°C | 2 |
| DSV838 | 818-838 | ||||
| GM3F | Bacteria | 8-24 | 45°C | 16 | |
| GM4R | 1492-1507 | ||||
| 341F(GC) | Bacteria | 341-357 | 65-55°Ca | 15 | |
| 518R | 518-534 |
a
These primers were used in a so-called “touchdown” protocol from 65°C to 55°C.