TABLE 1.
Strains and plasmids used
| Strain or plasmid | Reference or source | Description |
|---|---|---|
| Strains | ||
| RN6390 | 18 | Laboratory strain that maintains its hemolytic pattern when propagated on sheep erythrocytes and has a genetic background similar to that of 8325-4 |
| RN4220 | 21 | Mutant of 8325-4 that accepts foreign DNA |
| ALC1435 | This study | RN6390 with pALC1420 (sar P1 promoter) |
| ALC1436 | This study | RN6390 with pALC1421 (sar P3 promoter) |
| ALC1437 | This study | RN6390 with pALC1422 (sar P2 promoter) |
| ALC1440 | This study | RN6390 with pALC1434 (vector) |
| Plasmids | ||
| pCRII | Invitrogen | E. coli PCR cloning vector |
| pSK236 | 15 | Shuttle vector containing pUC19 cloned into the HindIII site of pC194 |
| pSPT181 | 17 | Shuttle vector |
| pALC70 | 16 | pSPT181 containing the entire sar locus with sarA, sarC, and sarB transcriptional units |
| pALC1434 | This study | Derivative of pSK236 containing a promoterless gfpUV gene preceded by a polylinker region |
| pLC4 | 23 | Transcriptional fusion vector containing a promoterless xylE reporter gene |
| pALC669 | This study | pCRII containing a 240-bp sar P1 promoter fragment (nucleotides 620 to 859) (2) |
| pALC669 | This study | pCRII containing a 162-bp sar P3 promoter fragment (nucleotides 364 to 525) (2) |
| pALC678 | This study | pCRII containing a 382-bp sar P2 promoter fragment (nucleotides 1 to 197 plus 185 bp upstream) (2) |
| pALC1420 | This study | pSK236 containing gfpUV preceded by the 240-bp sar P1 promoter fragment |
| pALC1421 | This study | pSK236 containing gfpUV preceded by the 162-bp sar P3 promoter fragment |
| pALC1422 | This study | pSK236 containing gfpUV preceded by the 382-bp sar P2 promoter fragment |