TABLE 1.
Purification and substrate specificities of G. trabeum glycosylhydrolases
| Enzyme (mass in kDa) | Amt of protein (μg) | Yield of activity in extract (%) | Sp act (U/mg) on substrate:
|
||||
|---|---|---|---|---|---|---|---|
| CMC | 4NPC | Xylan | PASC | Avicel | |||
| Extract | 11,600 | 100 | 27,000 | 4,900 | 26,000 | 670 | 7.3c |
| Cel5A (42) | 82 | 3.1a | 120,000 | 65 | 770 | 54 | 4.5d |
| Xyn10A (39) | 123 | 2.6b | 950 | 12,000 | 390,000 | 9.2 | 1.8 |
| Cel12A (28) | 4 | 0.1a | 42,000 | 69 | 880 | 49 | -e |
a
Percent of CMCase activity in the crude extract.
b
Percent of 4NPCase activity in the crude extract.
c
Mean specific activity of five separate preparations (standard deviation = 0.8 U/mg).
d
Mean specific activity of three separate preparations (standard deviation = 0.4 U/mg).
e
-, not determined.