. 2005 May;71(5):2737–2747. doi: 10.1128/AEM.71.5.2737-2747.2005

TABLE 2.

Changes (n fold) in transcript levels of A. nidulans secretion-related genes and putative yeast orthologs due to recombinant protein secretion and/or induction of the UPR with DTTh

Function	Yeast gene	A. nidulans ORF	ID or source	Change (fold)^e
Function	Yeast gene	A. nidulans ORF	ID or source	Chemostat	DTT (1h)
Protein folding/UPR
ER chaperone *bipA*	KAR2	AN2062.2	2000Sep131300_3520^a	1.31^e	3.90^e
			2000Sep131300_240^a	1.21^e	2.64^e
Protein disulfide isomerase *pdiA*^h	PDI1	AN7436.2	PCR	3.24^e	1.33^e
			2000Sep131300_1629^a	−1.01	1.30^e
Protein disulfide isomerase *prpA*	−^j	AN0248.2	PCR	2.57^e	1.06
Protein disulfide isomerase *tigA*	−^j	AN0075.2	PCR	1.49^e	−1.24^e
Calnexin—chaperone, folding *clxA*	CNE1	AN3592.2	2000Sep131300_3254^a	1.00	−1.02
Chaperone of the ER lumen	CER1	AN0847.2	−^j	−^j	−^j
Cyclophilin	CPR1	AN8605.2	2000Sep131300_1548^a	1.02	1.79^e
Cyclophilin *cypA*	CPR3	AN3814.2	pncs916aG10.SP6^a	1.21^e	1.27^e
Cyclophilin (cis-trans)	CPR8	AN4467.2	*cypB* (AF107254)^d	−^j	−^j
Peptidyl-prolyl cis-trans isomerase D	CPR5
Peptidyl-prolyl cis-trans isomerase	CPR2
Member of the cyclophilin family	CPR6	AN4583.2	pncs905aE03.SP6^a	1.37^e	4.71^e
Member of the cyclophilin family	CPR7		N12F7 SP6^a	1.23^e	1.58^e
Peptidyl-prolyl cis-trans isomerase precursor	CPR4		Contig 111^b	−^j	−^j
PPlase, FK506-binding protein	FPR1	AN3598.2	2000Sep131300_1483^a	−1.02	−1.38^e
FK506/rapamycin-binding protein of the ER	FPR2	AN8343.2	−^j	−^j	−^j
Proline cis-trans isomerase	FPR3	AN3908.2	k0f12a1.f1^b	−^j	−^j
Nucleolar peptidyl-prolyl cis-trans isomerase	FPR4
Mitotic peptidyl-prolyl cis-trans isomerase *pinA*	PIN1	AN6145.2	PCR	1.68^e	1.99^e
Stress response transcription factor *srrA*	SKN7	AN3688.2	2000Sep131300_325^a	1.46^e	−^j
Heat shock protein, 70 kDa	SSA1	AN5129.2	N8G4.SP6^a	1.36^e	1.52^e
	SSA2		SETIIpL.3-B10^a	1.21^e	2.03^e
	SSA3		10_2_C5.SP6^a	1.27^e	1.30^e
	SSA4		N5G5.SP6^a	1.17	1.23^e
Heat shock protein, 70 kDa	SSB1	AN4616.2	Pncs916aH11^a	−^j	−^j
Heat shock protein, 70 kDa, *hscA*	SSB2	X98931 ^c	PCR	5.96^e	2.07^e
Heat shock protein, 70 kDa	ECM10	AN6010.2	Pncs920aB09^a	1.37^e	1.14^e
Heat shock protein, 60 kDa	HSP60	AN6089.2	N15GE11.SP6^a	1.34^e	2.09^e
			N13C11.SP6^a	1.37^e	2.00^e
			N5G7.SP6^a	1.21^e	1.86^e
			SETIPL2G2.SP6^a	1.69^e	1.72^e
Heat shock protein, 30 kDa	−^j	AN5781.2	N14GA2.SP6^a	1.32^e	2.42^e
			10_1_C8^a	1.30^e	1.21^e
UPR transcription factor *hacA*	HAC1	AN9397.2	PCR	3.24^e	1.73^e
Serine/threonine protein kinase UPR	IRE1	AN0235.2	2000Sep131300_4522^a	−1.04	−1.16
Translocation
Translocon (alpha subunit)	SEC61	AN7721.2	Contig 212^b	−^j	−^j
Translocon (beta subunit)	SSS1	AN4589.2	−^j	−^j	−^j
Translocon (gamma subunit)	SBH1	AN0417.2	−^j	−^j	−^j
ER protein-translocation complex subunit	SEC62	AN6269.2	−^j	−^j	−^j
ER protein-translocation complex subunit	SEC63	AN0834.2	2000Sep131300_564^a	1.04	5.47^e
ER protein-translocation complex subunit	SEC71	AN1442.2	c8a09a1.f1^b	−^j	−^j
ER protein-translocation complex subunit	SEC72	AN7623.2	−^j	−^j	−^j
Signal sequence processing protein	SEC11	AN3126.2	−^j	−^j	−^j
19-kDa signal recognition particle	SEC65	AN0643.2	−^j	−^j	−^j
Signal sequence receptor alpha subunit	SRP101	AN6627.2	−^j	−^j	−^j
Signal recognition particle protein *srpA*	SRP54	AN8246.2	Contig 1155^b	−^j	−^j
Vacuolar protein sorting
Sec1-like protein (Golgi to endosome) *vpsA*	VPS45	AN6531.2	f5f09a1.r1^b	−^j	−^j
VPS plasma membrane to endosome *sagA*	END3	AN1023.2	PCR	−1.01	1.20^e
VPS (Golgi to plasma membrane)	SEC1	AN4724.2	−^j	−^j	−^j
VPS (endosome to vacuole)	VPS4	AN3061.2	−^j	−^j	−^j
VPS (Golgi to vacuole)	VPS41	AN4876.2	2000Sep131300_2938^a	1.06	1.32^e
VPS (vacuolar sorting protein)	VPS10	AN6118.2	−^j	−^j	−^j
Probable VPS,^gS. pombe (T50328)ⁱ	−^f	AN0854.2	SETIPL3G1.SP6^a	1.23^e	1.27^e
Sorting nexin, retrieval from endosomes	SNX41	AN6351.2	pncs902aB07.SP6^a	3.23^e	1.24^e
Probable vacuolar sorting protein *hbrA*	VPS33	AN2418.2	PCR	1.20^e	−^j
Protein degradation
ER degradation	DER1	AN8852.2	y4h09a1.f1^b	−^j	−^j
Ubiquitin-conjugating enzyme (UBC7 ortholog)	UBC7	AN5351.2	−^j	−^j	−^j
Ubiquitin-conjugating enzyme (UBC7 paralog)	−^j	AN8258.2	Contig 1112^b	−^j	−^j
Zinc finger for ER degradation E3	HRD1	AN1488.2	Contig 794^b	−^j	−^j
HMG-CoA^g reductase degradation	HRD3	AN0810.2	pncs911aB01.SP6^a	1.49^e	1.53^e
Ubiquitin-specific protease	DOA4	AN2072.2	−^j	−^j	−^j
E2 ubiquitin-conjugating enzyme—peroxin	PEX4	AN8258.2	−^j	−^j	−^j
Ubiquitin-activating enzyme	UBA1	AN2174.2	2000Sep131300_1506^a	1.02	1.87^e
26S proteasome regulatory subunit	RPT3	AN2904.2	2000Sep131300_1182^a	1.05	1.31^e
E2 ubiquitin-conjugating enzyme	UBC12	AN5344.2	2000Sep131300_790^a	1.07	1.01
Cellular export and secretion
Reticulocyte binding protein	SEC7	AN6709.2	−^j	−^j	−^j
Secretory component protein, putative	SHR3	AN1845.2	2000Sep131300_3678^a	1.00	1.27^e
Secretory pathway GDP-dissociation inhibitor	GDI1	AN5895.2	−^j	−^j	−^j
Putative myo-inositol-1-phosphate synthase	INO1	AN7625.2	2000Sep131300_3013^a	1.22^e	−1.29^e
			N1C4.SP6^a	1.22^e	−1.29^e
Cytosolic factor phosphatidylinositol transfer	SEC14	AN0243.2	−^j	−^j	−^j
Putative secretory component nsfA	SEC18	AN3098.2	pncs902aE06.SP6^a	1.20^e	1.24^e
Involved in nonclassical protein export pathway	NCE102	AN7683.2	2000Sep131300_1208^a	8.90^e	−^j
Secretory pathway Ca²⁺-ATPase pmrA	PMR1	AN7464.2	−^j	−^j	−^j
Glycosylation/modification
Oligosaccharyltransferase beta subunit	WBP1	AN4683.2	−^j	−^j	−^j
Oligosaccharyltransferase delta subunit	SWP1	AN1683.2	−^j	−^j	−^j
Oligosaccharyltransferase epsilon subunit	OST2	AN4031.2	2000Sep131300_883^a	−1.02	−1.25
Required for asparagine-linked glycosylation	ALG11	AN5725.2	aD02.b1.SP6^a	1.06	−1.26
Mannosyltransferase	ALG2	AN6874.2	2000Sep131300_4144^a	1.01	−5.64^e
Regulates mannosylphosphorylation	MNN4	AN4030.2	2000Sep131300_2411^a	1.01	1.02
Mannose-1-phosphate guanyltransferase	PSA1	AN1911.2	contig 627^b	1.22^e	−^j
Vesicle trafficking/transport
GTPase (ER to Golgi) sarA	SAR1	AN0411.2	2000Sep131300_2639^a	1.02	1.00
GTPase (ER to golgi Rab2 homolog) srgD	rab2^f	AN5106.2	2000Sep131300_2624^a	1.06	1.35^e
GTPase (cis to medial Golgi) srgB	YPT1	AN4281.2	PCR	1.22^e	1.43^e
GTPase (Golgi to plasma membrane) srgA	SEC4	AN6974.2	PCR	1.70^e	1.38^e
GTPase (intra-Golgi) srgE	YPT31	AN0347.2	PCR	1.49^e	2.27^e
	YPT32
GTPase (Golgi to vacuole) *srgC*	YPT6	AN7602.2	−^j	−^j	−^j
GTPase (late endosome to vacuole) avaA/srgF	YPT7	AN0089.2	−^j	−^j	−^j
GTPase (plasma membrane to endosome) srgG	YPT51	AN4915.2	2000Sep131300_3122^a	−1.05	−1.22^e
	YPT53
GTPase (early to late endosome) srgH	YPT52	AN3842.2	N8G5.SP6^a	1.24^e	−1.03
GTPase (late endosome to plasma membrane) srgL	rab11^f	AN0069.2	−^j	−^j	−^j
Protein transport protein	SEC13	AN4317.2	pncs912aF05.SP6^a	1.04	1.07
Targeting and fusion of ER-Golgi vesicles	TRS120	AN6533.2	2000Sep131300_717^a	1.24^e	−1.29^e
ER lumen protein-retaining receptor	ERD2	AN9415.2	pncs920aA07.SP6^a	−1.02	−1.29
Coatomer complex beta chain of vesicles	SEC26	AN1177.2	2000Sep131300_2823^a	−1.03	1.05
Coatomer complex beta chain of vesicles	SEC27	AN5972.2	pncs917aB12.SP6^a	12.82^e	1.46^e
ADP-ribosylation factor-like protein, ras	ARL3	AN1126.2	2000Sep131300_1470^a	−1.06	−1.26^e
Putative syntaxin (T-SNARE) protein (ssoA?)	SSO1	AN3416.2	2000Sep131300_1894^a	−1.08	−1.22^e
	SSO2
Lipid/inositol metabolism
sn-1,2-Diacylglycerol ethanolamine	EPT1	AN4778.2	pncs906aE03.SP6^a	1.28^e	−1.25^e
Fatty acyltransferase	SLC1	AN6139.2	2000Sep131300_2940^a	−1.05	1.69^e
3-Hydroxy-3-methylglutaryl-coenzyme A reductase 2	HMG2	AN3817.2	2000Sep131300_2868^a	1.27^e	−1.84^e
Cell wall biogenesis
Involved in cell wall biogenesis, architecture	ECM3	AN0930.2	N1H1.SP6^a	1.52^e	1.20^e
P-type ATPase—unknown function	SPF1	AN3146.2	pncs919aG12.SP6^a	1.34^e	1.07
Strong similarity to Chs6p FMP50	FMP50	AN3122.2	pncs913aA03.SP6^a	−1.01	3.39^e

Pipeonline (http://bioinfo.okstate.edu/pipeonline).

Oklahoma cDNA and EST sequencing database (http://www.genome.ou.edu/fungal.html).

Not predicted by automated annotation.

GenBank accession number.

Significant fold change (43).

No homolog in S. cerevisiae.

VPS, vacuolar protein sorting.

Boldface type indicates previously cloned Aspergillus genes.

ⁱ

Schizosaccharomyces pombe.

−, no data available.