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HPLC analysis of penicillin G degradation product. AhlM was reacted with 2 mM penicillin G for 1 h and separated by HPLC after OPA derivation (bottom). 6-Aminopenicillanic acid was used as the standard (top). The elution gradient was made as described in the legend to Fig. 3. The hatch marker corresponds to changes in the mobile phase ratios during the course of the run. AU, absorbance units.
