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Substrate specificities of AhlM against AHLsa
| Substrate | Sp act (pmol · min−1 · μg−1) | Relative activity (%) |
|---|---|---|
| C4-HSL | 0.0 ± 0.1 | 0 |
| C6-HSL | 1.1 ± 0.2 | 1.0 |
| C8-HSL | 105.2 ± 12.0 | 100.0 |
| C10-HSL | 61.1 ± 6.0 | 58.1 |
| C12-HSL | NDb | ND |
| 3-oxo-C6-HSL | 1.4 ± 0.1 | 1.3 |
| 3-oxo-C8-HSL | 15.4 ± 1.5 | 14.6 |
| 3-oxo-C12-HSL | 50.9 ± 1.4 | 48.4 |
The data present the mean values of three independent experiments.
ND, not determined due to poor solubility of the substrate.
