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. 2024 Feb 5;15:1332175. doi: 10.3389/fmicb.2024.1332175

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Copyright © 2024 Andreu, Agúndez,, López-Guerrero and Bello-Morales.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of AP2M1 shRNA knockdown efficiency in PK15 cells. PK15 cells were transfected with a pool of shRNA to KD the expression of AP2M1. KD efficiency was checked by both Western blot and RT-qPCR. (A) Western blot analysis of total cell lysates subjected to SDS-PAGE showing AP2M1 for each condition. β-actin was chosen as protein loading control. Values of immunoblot quantification are reported as the mean ± S.D. (n = 3); *p < 0.05. (B) qPCR amplification of AP2M1 mRNA from AP2M1 A6, B3, and C1 shRNA-treated PK15 cells in comparison to the non-targeted control. mRNA level is relative to β-actin (ACTB) control (n = 6, mean ± S.D.). *p < 0.05 was considered as statistically significant compared to the non-targeting shRNA-transfected cells.