FIG. 3.

Agarose gel of restriction endonuclease linearization of plasmids using EcoRV (except that AccI was used to linearize plasmid from HS1858) (A) and Southern hybridization analysis of gel shown in panel A transferred to nitrocellulose membrane and probed with a DIG-labeled PCR product containing the tet(B) gene (B). Lanes 1, pHS-Tet; lanes 2, HS226 plasmid; lanes 3, HS1857 plasmid; lanes 4, HS1858 plasmid; lanes 5, HS1859 plasmid; lanes 6, HS1861 plasmid. DNA fragment sizes are indicated in kb.