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(A) TMPK activity in cell extracts of doxycycline-induced Tet-On-TMPK RKO cells. The activity of the enzyme TK was used as a control. (B) Expression of TMPK in Tet-On-TMPK RKO cells, as detected by Western blotting. Actin was used as the internal control. (C) Correlation of TMPK activity and its protein amount quantitated with a densitometer.
