FIG. 1.

omp1 and pCT PCR of strains C599 and E/UW5. Primers designed for omp1 are located 108 bp upstream of the ATG initiator and ∼80 bp downstream of the TAA terminator, resulting in a PCR product of approximately 1,250 bp. Primers designed for pCT are located in ORF8, producing a PCR product of approximately 720 bp. Lanes: 1, 1-kb DNA size standards (Life Technologies, Gibco BRL); 2, omp1 PCR of C599; 3, omp1 PCR of E/UW5; 4, pCT PCR of C599; 5, pCT PCR of E/UW5; 6, negative omp1 control PCR (no template); 7, negative pCT control PCR (no template). Black-and-white digital images were captured with an Alpha Innotech Alpha-Imager 2000 and annotated by using Adobe Photoshop and Freehand V.8 for Power Macintosh.