FIG. 2.

Southern hybridization of BamHI-digested C599 and E/UW5 total DNA probed with DIG-labeled omp1 PCR product (A) and DIG-labeled pCT (B). Lanes: 1, DIG II marker bands at (from top to bottom) 23, 9, 6, 4, 2.3, and 2 kb; 2, 1 μg of E/UW5 BamHI-digested DNA; 3, 1 μg of C599 BamHI-digested DNA; 4, 0.5 μg of E/UW5 BamHI-digested DNA; 5, 0.5 μg of C599 BamHI-digested DNA; 6, BamHI-linearized pCT (7.5 kb); 7, omp1 PCR product (1,250 bp). Southern blot X-ray images were scanned at 400 dots per inch with a UMax Powerbook scanner. The images were then combined into one photo by using Adobe Photoshop and Freehand V.8 for Power Macintosh.