FIG. 3.
Inhibition of HCV CRC replicase activity by nucleoside triphosphate analogs. The effect of 2′-C-Me-ATP and 2′-O-Me-CTP were tested in an in vitro reaction with HCV replicase complexes isolated from HBI10A and HBIII27 replicon cells as described in Materials and Methods. RNA products were purified by phenol extraction and ethanol precipitation, electrophoresed on a glyoxal denaturing agarose (0.7%) gel (A), and quantitated by phosphorimager analysis. The IC50s determined for each NTP in the replicase and NS5B polymerase reactions are summarized in panel B.