TABLE 2.

Antiviral effect and intracellular metabolite levels

Compound	Replicon EC50 (μM)a for cell type:		pmol of NTP/106 cellsb of type:		pmol of N/106 cellsc of type:
	HBI10A	HBIII27	HBI10A	HBIII27	HBI10A	HBIII27
2′-O-Me-cytidine	21.2	>100	0.2	0.010	1.3	0.5
2′-O-Me-cytidine (SATE)	3.2	4.0	5.8	1.0	22.5	15.0
2′-C-Me-cytidine	4.8	NDd	0.8	ND	3.0	ND

^aReplicon activity was determined 24 h post-compound addition by in situ RPA.

^bTotal NTP measured at 24 h.

^cTotal radiolabeled metabolites measured at 24 h.

^dND, not determined.